![]() ![]() Protein turnover is the continual, metabolic process regulating tissue mass and function, and reflects the balance between rates of protein synthesis and breakdown. Protein metabolism, protein status, 15N-glycine, stable isotope tracer, nutrition, military training, physiological stress Introduction Application of this method may improve our understanding of protein kinetics during conditions of high physiological stress in free-living environments such as military training. The end-product method using 15N-glycine is a practical method for measuring whole-body protein turnover in the field over short (24 h) time frames and has been used effectively in recent military field research. However, the end-product method can also be used to measure acute (over 9–24 h) whole-body protein turnover noninvasively by ingesting 15N-glycine, or equivalent isotope tracers, and collecting urine samples. Both methods require invasive procedures such as the infusion of amino acid tracers and muscle biopsies to assess the uptake of the tracer into tissue. The precursor method is the gold-standard for measuring acute (over several hours) skeletal muscle protein turnover, whereas the end-product method measures chronic (over several weeks) skeletal muscle protein turnover and provides the opportunity to monitor free-living activities. The 2 primary approaches for measuring protein turnover are based on precursor- and end-product methods. We undertook a narrative review of published literature with the aim of reviewing the suitability of, and advancements in, stable isotope methods for measuring protein turnover in field research. Evaluating protein turnover in free-living environments, such as military training, can help inform protein requirements. skeletal muscle only) level using stable isotope methods. aggregated across all body proteins) or tissue (e.g. Protein turnover reflects the continual synthesis and breakdown of body proteins, and can be measured at a whole-body (i.e. ![]()
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